Product Name :
CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55]
Predicted band size :
158 kDa
Observed band size :
158 kDa
Synonyms:
Cas9 antibody CRISPR-associated endonuclease Cas9/Csn1 antibody CRISPR-Cas9/Csn1 antibody csn1 antibody SpyCas9 antibody
Function :
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3′-5′ exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Recombinant protein within Streptococcus pyogenes cas9 aa 730-990 / 1368.
Species reactivity:
Streptococcus pyogenes
Verify application:
WB, IF-Cell, IF-Tissue, IHC-P, FC
Coupling :
unconjugated
Clone number:
JM11-55
RRID:
AB_3070439
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.{{1,2-Distearoyl-sn-glycero-3-phosphorylcholine} web|{1,2-Distearoyl-sn-glycero-3-phosphorylcholine} Metabolic Enzyme/Protease|{1,2-Distearoyl-sn-glycero-3-phosphorylcholine} Technical Information|{1,2-Distearoyl-sn-glycero-3-phosphorylcholine} In Vivo|{1,2-Distearoyl-sn-glycero-3-phosphorylcholine} manufacturer|{1,2-Distearoyl-sn-glycero-3-phosphorylcholine} Epigenetics} Aliquot store at -20℃ or -80℃.{{Vorinostat} medchemexpress|{Vorinostat} Apoptosis|{Vorinostat} Purity & Documentation|{Vorinostat} Data Sheet|{Vorinostat} custom synthesis|{Vorinostat} Epigenetic Reader Domain} Avoid repeated freeze / thaw cycles.PMID:29844565
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
158 kDa
Positive control :
293T transfected with Cas9 cell lysates.