Name :
APLN Protein
Description :
Macrophages play key roles during cardiovascular diseases (CVD) and their related complications.Apelin (APLN) is a key molecule, whose roles during CVD have been documented previously. Therefore, it has been hypothesized that APLN may perform its roles via modulation of macrophages.
Species :
Mouse
Uniprotkb :
HEK293
Tag :
C-hFc-Avi
Synonyms :
APEL, APJ endogenous ligand, Apelin
Construction :
Recombinant Biotinylated Mouse APLN Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Val23-Phe77.[Accession |Q9R0R4]
Protein Purity :
> 95% as determined by Tris-Bis PAGE
Molecular Weight :
The protein has a predicted MW of 34.81 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
Endotoxin :
Less than 1EU per μg by the LAL method.
Formulatione :
Lyophilized from 0.22μm filtered solution in PBS, 0.2M Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage :
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background :
Macrophages play key roles during cardiovascular diseases (CVD) and their related complications.Apelin (APLN) is a key molecule, whose roles during CVD have been documented previously. Therefore, it has been hypothesized that APLN may perform its roles via modulation of macrophages.
References and Literature :
1. Arababadi MK, et al. APLN/APJ pathway: The key regulator of macrophage functions. Life Sci. 2019 Sep 1;232:116645. doi: 10.1016/j.lfs.2019.116645. Epub 2019 Jul 10. PMID: 31299236.
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